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KMID : 1007520160250041115
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Food Science and Biotechnology
2016 Volume.25 No. 4 p.1115 ~ p.1122
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A Monascus pilosus MS-1 strain with high-yield monacolin K but no citrinin
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Feng Yanli
Chen Wanping
Chen Fusheng
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Abstract
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Monacolin K (MK) produced by Monascus spp. is mostly used to regulate cholesterol level, while it can be contaminated by citrinin, a mycotoxin yielded by some Monascus strains. To develop production of citrinin-free MK, an M. sp. MS-1 strain, identified as M. pilosus by morphologies and internal transcribed spacer sequences analysis, was isolated from red fermented rice. After 14 days of fermentation, yields of citrinin-free MK could be up to 0.58 mg/mL and 16.45 mg/g in liquid- and solid-state fermentation products under optimal conditions, respectively. Further determination revealed that no citrinin biosynthesis related genes such as ctnA, pksCT, ctnE, and ctnR were detected. Thus, HPLC combined with citrinin-related gene analyses can be used for rapid screening of non-citrinin production Monascus strains.
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KEYWORD
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monacolin K, citrinin, Monascus pilosus, gene cluster, identification
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